Quantitative determination of curcumin, demethoxycurcumin and bisdemethoxycurcumin from dietary supplements using HPLC-UV

Abstract

A simple, sensitive and reliable method was developed and applied to determine curcumin, demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC) (curcuminoid) in dietary supplements by High-Performance Liquid Chromatography using UV detector (HPLC-UV). The method was validated for linearity, repeatability, reproducibility, limits of detection and limits of quantification. The limits of detection and limits of quantification of curcuminoids were found to be 10 mg/kg and 40 mg/kg, respectively. The calibration curve showed good linearity for the three compounds (R2 > 0,999). The average recovery rates of curcuminoid at three spiked levels ranged from 90.3% to 106.7% and the relative standard deviations were less than 2.0%. The method has also been successfully applied to analyze curcuminoid in the real samples collected in Ho Chi Minh City.