Evaluation of microbiological and heavy metal safety assessment of Phellinus linteus mass powder from submerged fermentation

Abstract

Phellinus linteus is known to be a medicinal mushroom which is very good to human health. They are used as medicinal mushroom in many Asian countries such as Japan, Korea, China, Thailand and Vietnam. Because of long time growth and over-havest by humans in natural conditions, they are artificially cultured in these countries. There were advances of liquid culture of mushroom such as time saving and scaleable in culture in comparison with culturing of mushroom in decaying wood. In this study, Phellinus linteus is cultured in optimum liquid medium, lyophilized, ground into fine powder and evaluated for microbiological and total of Asen and Lead to ensure the biomass product will meet safety standards for users. Results are as follows: Total of anaerobic bacteria 2,2 × 10­⁴ CFU/g, Coliforms< 1,0 × 10¹ CFU/g, Coagulase positive with Staphylococci <1,0×10¹ CFU/g, total amount of yeast-mold < 1,0 × 10¹ CFU/g, not detected of E.coli, Clostridium perfringens and Salmonella spp.. Furthermore, we did not find any Asen and Lead which are supposed to remain in our sample.