Research for toxicity and determination IC50 of tea leaf extracts on cervical cancer cell and breast cancer cell MCF7 by in vitro

Abstract

After extracting tea leaves with methanol 80% (acidified with HCl), the extraction was purified through two phases: the first stage used the column of silica gel with gradient elution, the second stage continued to be isolated on Sephadex LH-20 column with isometric elution mode, K powder was obtained. The test of toxicity of K powder on two MCF37 breast cancer cell lines and Hela cervical cancer was carried out and obtained the following results: IC50 of the composition with the line MCF37 was 31.62 µg/mL and with the line Hela was 50.1 µg/mL. Compared with the positive control group of Taxol showed that the efficacy of the obtained product was lower. However, this is a new direction in the use of drugs derived from plants in general and tea leaves in particular.