INVESTIGATION OF THE CONTENTS OF ESSENTIAL OIL, EXTRACTABLE MATERIALS, Z-LIGUSTILIDE AND FERULIC ACID FROM ANGELICA ACUTILOBA AND THEIR ANTIFUNGAL ACTIVITY AGAINST PLANT FUNGI

Abstract

Angelica acutiloba was introduced to Vietnam and popularly grown in the Northwest provinces such as Lao Cai, Hoa Binh, Lai Chau and the Central Highlands such as Da
Lat, Lam Dong. In traditional medicine, it is considered female ginseng and is used to treat endocrine diseases, flatulence and joint diseases, skin diseases. In addition, A.
acutiloba has antibacterial effects and can treat abdominal pain, muscle spasms, and relieve symptoms of bronchitis. In this study, the content of essential oils, the content
of extracts obtained with different solvents (extract residues), the content of two main active ingredients Z-ligustilide and ferulic acid in the roots of the samples of A.
acutiloba grown in the province Lao Cai has been surveyed and compared with the provisions of the Vietnamese Pharmacopoeia V. The essential oil content of A. acutiloba
root is between 0.06 - 0.19%. The content of extracts is determined to be between 10.85 - 35.78%. The Z-ligustilide content was in the range 83.33 - 198.45µg/g (wt/dwt)
and ferulic acid content was from 130.79 to 488.05µg/g (wt/dwt) based on the analysis of HPLC. Test results for three fungal strains of plant pathogens, Sclerotium rolfsii,
Fusarium oxysporum and Colletotrichum orbiculare, showed that the crude extracts and essential oils were relatively strong against the tested strains of fungi in vitro. At a
concentration of 500µg/mL, A. acutiloba root essential oil strongly inhibited mycelium growth of S. rolfsii (100%), F. oxysporum (82 - 84%) and C. orbiculare (81 - 100%) at
2 - 4 days after treatment. The residues from A. acutiloba roots also showed different inhibitory abilities on the growth of tested fungi at 1000µg/mL.