SCREENING AND EVALUATION OF ACETYL ESTERASE AND FERULOYL ESTERASE HYDROLYTIC ACTIVITY FROM SOME FUNGAL STRAINS ISOLATED IN NORTHERN TROPICAL RAIN FOREST

Abstract

Lignocellulose is the biomass source that has a low commercial value, but it is economically
important when used as a raw material for applications in biotechnology. Fungi are known to be able
to biosynthesize various hydrolytic enzymes to effectively attack lignocellulose structures.
Carbohydrate esterase (CE) hydrolysis enzyme is of role importance in the hydrolysis of the
lignocelluloses, which represents a group of hydrolases enzyme for the separation or formation of
saturated and aromatic esters. In which, acetyl esterase (AE) and feruloyl esterase (FAE) break down
branch chains of the cell wall polysaccharide structure to cut the interaction between xylan chains and
xylan with lignin to separate lignin from the lignocellulosic structure. In this study, 23 fungal strains
which isolated in the Northern Tropical Rain Forest (Cuc Phuong National Park) were screened for FAE
and AE enzyme activity. In which, 20/23 fungal strains have been identified exhibit activity from 01 to
02 enzymes. The strains showed the highest AE and FAE activity were identified as Lentinus brumalis
GNB10 (≈ 2353.8 U/L), and Xylaria sp. GNB3 (D = 20mm), respectively.