ISOLATION AND SELECTION OF BACILLUS BACTERIA FROM SHRIMP PONDS WITH BIOACCUMULATION POTENTIAL

Abstract

The research on biofloc generation is one directions aimed to improve the
quality of pond water as well as the effective use of feed in aquacultural ponds.
In biofloc technology, it is very important that the suspended solids in pond
water including microbial cells are flocculated in the form of loosely aggregated
particles. Bioflocculation by bacteria probably plays crucial role in the formation
of these particles. In this study, 03 strains of Bacillus sp. B15, B5, and B12 showed
the best bioflocculating capability among 20 strains isolated. These strains were
used to determine the effects of some factors on the bioflocculating
effectiveness. The results showed that the most suitable medium for B15 and B5
strains were glucose, yeast extract, and CaCl2; B12 strain was glucose, glutamate,
(NH4)2SO4 for more than 60% flocculating rate with kaolin solution after 5
minutes to settle at pH 7 and 1% (v/v) of bacterial broth. This is the initial
research towards the application of bio-products to treat shrimp pond water
using biofloc technology.