Molecular identification and survey of antibiotic resistance properties of streptococcus bacteria causing acute pharyngitis in humans

Abstract

"Molecular identification and survey of antibiotic resistance properties of Streptococcus bacteria causing acute pharyngitis in humans" with the objective of isolating, identifying molecular and investigating antibiotic resistance of some strains of Streptococcus bacteria causing acute pharyngitis in humans. From 50 samples after culture, 125 bacterial strains were isolated. Out of 125 strains, Gram stained and observed morphology 44/125 strains of streptococci of which 38/44 strains were Gram (+), 6/44 were Gram (-) proving that 38/44 strains are likely to be Streptococcus strains. From 38 Gram - positive streptococcal strains, randomly selected 4 strains were conducted for DNA extraction, PCR run and gen 16S rRNA sequencing (04 samples) see the bacterial strains studied in the group Streptococcus and share the closest similarity with strains of S. mitis, S. oralis, S. pseudopneumoniae. Survey results of antibiotic resistance showed that all 4 strains 155, 157, 158, 159 are resistant to 3 types of antibiotics: Amoxicillin, Azithromycin, Cephalexin and sensitive to Clindamycin.