Quantitative determination of arbutin in whitening face cream by High Performance Liquid Chromatography (HPLC)
Abstract
Arbutin is considered a crucial ingredient in cosmetic preparations thanks to its safe whitening mechanism. In Vietnam, studies aiming to develop a process for arbutin quantification have remained lacking. Hence, quantitative analysis of arbutin by chromatography with a photodiode array detector was carried out under chromatographic screening conditions and a procedure for quantification of arbutin in skin whitening cream was developed. The chromatography system performed with a LiChrospher® 100 RP-18 chromatography column (25 cm ´ 4 mm ´ 5 µm); PDA detector placed in the wavelength range from 190 – 800 nm, taking chromatogram at 283 nm; mobile phase H2O:ACN (80:20); flow rate 0,5 mL/min, sample injection volume 40 µL; column temperature set at ambient room air. The quantification procedure validated thesystem compatibility, specificity, linearity, accuracy and precision, LOD and LOQ according to the Content and Methodology Guidelines of the International Conference on Harmonization (ICH). In conclusion, the present study has successfully developped an Arbutin quantitative analysis which is suitable for analyzing commercialized whitening creams containing arbutin.
