Rapid detection of classical swine fever virus (CSFV) by reverse-transcription loop-mediated isothermal amplification method (RT-LAMP)

Abstract

    Classic swine fever (CSF) is one of the most serious infectious diseases in pigs, caused by CSFV virus, and has the following characteristics: widespread spread, high fever, and a high swine sickness and death rate in the area. As a result, rapid and accurate disease detection is a crucial challenge in disease control. The RT-LAMP − an isothermal amplification method was used in this study to detect the specific sequence at the CSFV RdRp gene. The optimized RT-LAMP procedure in this study could detect the CSFV target sequence with a detection limit of 10^−0.5 TCID₅₀/mL; the reaction was performed at a constant temperature (63 ℃) for 35 minutes. The naked eye could quickly read the results through the color of the reaction, which indicates that this method has a high applicability for the rapid detection of diseases at the point-of-care setting.