Isolation of Chemical Composition from Hedyotis Dichotoma Koen.Ex Roth, Rubiaceae

Abstract

     Pharmaceutical chemistry studies show that the genus Hedyotis contains many organic compounds with biological activities such as triterpen, antraquinon, steroid, glicosid, flavonoid, iridoid and alkaloid. In this study, Hedyotis dichotoma Koen.Ex Roth, Rubiaceae were picked in Binh Phuoc province and studied with the aim of finding out the chemical compostion by isolating and determining the struture of the chemical component. 6.50 kg of Hedyotis dichotoma Koen.Ex Roth trunk after being harvested, was dried, cut finely and soaked in ethanol to prepare an ethanol gel. This gel was extracted from the solid phase on silicagel to obtain four different gels: petrolium ether, dicloromethane, ethyl acetate and methanol. Dicloromethane gel was studied via column chromatography with a variety of solvents from less polar to polar. Two product compounds were isolated and one isolation procedure was built. The compound structures were determinized through analysis of their NMR-spectroscopic data with ¹H-NMR, ¹³C-NMR, 2D-NMR HSQC, HMBC and they were named acid 4-hydroxy-3-metoxybenzoic (Dichoto.Diclo1) and (22E)-5a-Stigmasta-7,22-dien-3b-ol (Dichoto.Diclo2). Research results show that the compound Dichoto.Diclo2 has the same struture as Spinasterol, which brings new information into pharmaceutical chemistry field of Hedyotis dichotoma Koen and increases its application value in real life. Furthermore, the obtained data will be valuable foundation for further study.