Lansoprazol optical analysis using mobile phase containing β-cyclodextrin derivatives

Abstract

     A high performance liquid chromatographic (HPLC) method was developed for the chiral separation of lansoprazol. The two enantiomers were separated on a Gemini NX C18 (150 mm  x 4.6 mm; 5 µm) column. The mobile phase was acetonitril – 10 mM phosphate buffer pH 2.5 containing 10,0mM SBE-β-CD (20:80) (v/v/v). UV detection was at 285 nm. Detection limit is 0.10 µg/mL; relative standard deviation of the method is less than 2 %; the quantification range is from 150 g/mL to 1200 g/mL; (n = 6); recovery rate is from 98 % to 102 %.