Investigation of the analgesic and anti-inflammatory effectes of extract from fruit of Musa acuminata Colla., Musaceae on white mice

Abstract

In Vietnam, in folklore, forest wild bananas (Musa acuminata Colla., Musaceae) are used traditionally for the management of many diseases such as arthritis, diarrhea, allergies... The objective of this study was to determine chemical composition, acute toxicity, analgesic and anti-inflammatory effects of extract from wild bananas collected in Tay Nguyen. The extract was obtained by thoroughly soaking wild banana fruit with 60% ethanol alcohol. The groups of active ingredients were quantified by the Ciuley method. In the acute toxicity test, gave the mice extract to drink with the maximum possible dose. The authors observed and recorded general behavioral changes, death rate of the mouse for 72 hours and up to for 14 days post-treatment. The central analgesic effect were investigated on the mouse tail dipping model and the peripheral analgesic effect - on the model of causing cramping abdominal pain by acetic acid 1 %. The anti-inflammatory effects were investigated on the mouse paw edema model with 1% carrageenan. Qualitative phytochemical analysis of the extract revealed the presence of many bioactive compounds such as free triterpenoids, flavonoids, tannins, proanthocyanosides, saponins, organic acids, polyuronics. The results showed that the maximum tolerated dose ( ) that could cause death was 26 g/kg (Musa acuminata Colla extract/mouse body weight). The  Musa acuminata Colla extract at doses of 1.3 g/kg and 2.6 g/kg mouse body weight had anti-inflammatory and peripheral analgesic effects but no central analgesic effect. The results will be useful for further research on natural preparations used in supporting of the treatment of chronic diseases as osteoarthritis.