Study on the chemical compounds of EtOAc fraction from the fruits of Solanum torvum Sw.

Abstract

    By thorough percolation 7.02 kg powder of Solanum torvum Sw. with 70 L of EtOH 96 % and then spinning condensate with pressure drop, we got 656.88 g of EtOH 96 % extract. From which, in fractional extraction, we revived 318.60 grams of cloroform extract and 15.75 g of EtOAc extract. And then 14 g EtOAc was isolated by rapid column chromatography in 5 fractions (I – V). At the IV fraction, a white crystals precipitated, which was determined by cleaning several times with cold CHCl₃, EtOAc, MeOH on the porous glass funnels respectively and dissolved with MeOH to obtain white needle-shaped crystals of S-1 (596.71 mg). The remaining yellow extract was isolated by Sephadex LH-20 column chromatography to obtain S-3          (18.46 mg). At the III fraction, S-2 (119.54 mg) was determined by classical column chromatography. Based on mass spectrometry (MS) data, nuclear magnetic resonance spectroscopy (1D-NMR, 2D-NMR) and the comparision with refference materials, was identified S-1 is neochlorogenin 6-O-α-L-rhamnopyranosyl-(1→3)-β-D-quinovopyranoside, S-2 is (25S)-6α-hydroxy-5α-spirostan-3-on-6-O-α-L-rhamnopyranosyl-(1→3)-β-D-quinovopyranoside, S-3 is Solanolactoside A.