Evaluation antibacterial activity of fractions from stem extract of Tinospora crispa (L.) Hook. f. & Thomson
Abstract
The aim of this study is to evaluate antibacterial activity of fractions from Tinospora crispa. Tinospora crispa has been used in folkloric medicine for curing malaria, coughing, digestive diseases, large intestinal inflammation, some cancers and diabetes. The extraction is fractionated by solvents with increasing polarity. On the other hand, this extraction is partioned with acidic water and base to concentrate alkaloids. There are totally 7 fractions accordingly named PĐ-hex, PĐ-K, PĐ-Cf2, PĐ-Cf1, PĐ-EtOAc, PĐ-BuOH và PĐ-N. With the help of disk-diffusion method, the 7 fractions are separately evaluated the ability inhibit the growth of some common bacterial species, including 3 gram-positive and 3 gram-negative ones. The result is that Tinospora crispa is able to restrain the spread of the 3 gram-positive species but not the 3 gram-negative species. Antibacterial subtances are distributed in 6 over 7 fractions tested, excluding PĐ-K. PĐ-K sample mainly contains strong basic alkaloid that are also water soluble. Especially, weak basic alkaloids in PĐ-Cf2 are able to restrict the growth of bacteria stronger than that of stronger basic alkaloids in PĐ-K.
