Optimal analytical conditions of chlorinated phenoxy acid herbicides in groundwater using ultra high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS)

Abstract

   The study presents the method of mass spectrometry with UPLC-MS/MS for analysis of residue of herbicides of the CPA compounds in river water samples, including 2.4-D, MCPA, MCPP, 2.4-DP, 2.4-DB, 2.4.5-T. We combined LC-MS/MS and ESI (-) methods with MRM recording mode to determine, using UPLC BEH RP18 Acquity column, 1.7μm, 2.1×100mm. We optimized the parameters of the mass spectra to determine the m/z ion fraction through a gradient run program with a 10μl injection volume, a column temperature of 40°C, a flow rate of 0.3ml.min^-1. The active phase consists of 0.01% formic acid and acetonitrile. Therefore, the above optimized chromatographic conditions are appropriate to quantify the CPA compounds.