Imperatorin standard application for quantification determination of imperatorin from Angelica in the market of HCM city

Abstract

   In this project, we practiced the combination method of Thin Layer Chromatography (TLC) and High-Performance Liquid Chromatography (HPLC) with PDA detector to determine Imperatorin presence and its quantification. Imperatorin was got from Angelica cataplasm samples at Binh Minh pharmaceutical company and in the market on Hai Thuong Lan Ong street. From experimental result, the Rf Value of the Standard Source comparing to the two examination samples by TLC were equivalent. Percents of Quantification of Imperatorin in the pharmaceutical sample were 0,84% and 0,37%. Percents of relative standard deviation (% RSD) in the pharmaceutical sample were 0,23÷0,47%. Therefore, the Accuracy and the Stableness of this method is high.