Determination of acute toxicity and investigate coagulation activity of Psychotria rubra aqueous extract

Abstract

In Viet Nam, Lau do (Psychotria rubra) is used for treatments, such as bleeding. This study was conducted for the purpose of determinating acute toxicity and evaluating the hemostatic and coagulation effects of Psychotria rubra aqueous extract. Lau do was collected at Quang Binh province, dried and extract to aqueous extract. The results showed that LD50 of aqueous extract is 25,01mg/kg. In in vivo model, extract of Psychotria rubra has hemostatic effect at 2 dosages: 2,50 and 1,25g/kg but doesn't has coagulation effect. In in vitro model, the extract in 3 concentrations: 0,5; 1,0; 2,0mg/ml does not change PT and APTT time compare to DMSO. This extract shows significant hemostatic effect but doesn't has coagulation effect. This is a highly potential medicinal plant for the treatment of bleeding diseases and should be investigated the others pharmacological effects.