Prevalence of antibiotic resistance genes (ARGs) in faecal sludge in Vietnam

Abstract

    The main goal of this study is to investigate the prevalence of 12 ARGs for 9 common antibiotics in faecal sludge samples collected, in Hanoi and Ho Chi Minh cities. We collected 26 faecal sludge samples: 2 of Hanoi city and 24 of Ho Chi Minh City. The existence of ARGs was tested using PCR in two ways: (1) directly with the sludge samples and (2) with the E. coli strains isolated from the samples. Our results showed that the prevalence of the tested ARGs in faecal sludge is significant isolated E. coli, the presence of ARGs in Hanoi city and Ho Chi Minh City was 0 and 44% for Streptomycin resistance; 50% and 40% Sulfonamide Resistance; 0 and 4% resistance to Erythromycin; 0 and 65% chloramphenicol resistance; 100% and 98% Tetracycline resistance; 50% and 16% Trimethoprim Resistance; 100% and 30% resistance to β-Lactams; 0 and 0 Gentamycin resistance. There was no Quinolone resistance gene for isolated E. coli in both cities.