Study on anti-inflammatory effects of some flavonoids isolated from wormwood (artemisia vulgaris – asteraceae) in mice

Abstract

    Wormwood is a promising herb to alleviate symptoms of inflammatory. In this research, we evaluate the anti-inflammatory effects of some flavonoids isolated from Wormwood: NC1(5,7-Dihydroxy-6,3‟,4‟-trimetoxy flavon),NC2 (5,7,3-Trihydroxy-6,4,5-trimetoxy flavon) to contribute to creating new materials from natural sources which have good therapeutic and few side effects. Materials: Two pure natural compounds: NC1,NC2 were isolated from Wormwood (Artemisia vulgaris - Asteraceae). Methods: The anti-inflammatory effects were studied by using carrageenan-induced paw edema model in mice. Paw volume of mice was measured before injecting carrageenan 1%. Three hours after carrageenan injection, measure paw volume again and calculate the edema percentage. Control group was given distil water, while experiment groups were given NC1, NC2 and positive control group received diclofenac 5mg/kg PO. The edema percentage was determined in the next day, and the procedure kept continue in 6 days after inflammation inducing. Two tested compounds have anti - inflammatory. The anti - inflammatory effect of NC2 is faster, stronger and more stable than NC1. The anti - inflammatory effect of NC2 is 117% respectively in comparison with diclofenac 5mg/kg. The research has successfully proved the anti-inflammatory effects of two pure natural compounds isolated from Wormwood (Artemisia vulgaris - Asteraceae), especially NC2 is a promising compound for anti - inflammatory drug research and development.