DEVELOPMENT OF A LATERAL FLOW IMMUNOCHROMATOGRAPHY TEST STRIP FOR RAPID DETECTION OF CHOLERA TOXIN

Abstract

Cholera toxin produced by Vibrio cholerae is one of the most common waterborne pathogens responsible for dysentery, especially in developing countries. Therefore, it is necessary to detect CT rapidly to control the quality of water and diagnose the disease caused by this pathogen. In this study, the test strip for CT rapid detection has been successfully developed based on immunochromatographic techniques. The concentration of antibodies on the control line and the test line were 1.3 µg/cm and 4 µg/cm, respectively. The suspension of gold nanoparticles 15±2 nm, OD₅₂₀=1 was prepared for conjugating with the capture antibody at a concentration of 200 µg/mL, pH 9. The resulted test strip enabled the detection of CT at 10 ng/mL within 10 min. The sensitivity and specificity of the test strip were 96.67% and 100%, respectively. These results are initial steps for developing biological kits to quickly detect pathogens in water and food.