THE INFLUENCE OF SOME FACTORS ON THE EFFICIENCY OF CodA GENE TRANSFORMATION INTO THE DT22 SOYBEAN VARIETY

Abstract

Soybean is a sensitive crop to abiotic stresses and is considered a poor tolerant plant to the adverse environmental factors. The application of gene transfer to improve tolerance to abiotic stresses in soybeans is being researched concerned. The efficiency of genetic transformation in soybeans not only depends on the genotype of each variety but is also influenced by factors such as damage manipulation, bacterial concentration, selective substances, growth stimulants... In this study, some factors affecting the efficiency of CodA gene transfer into the soybean variety DT22 were investigated. The results showed that using 3.0 mg/l phosphinothricin (ppt) in the SIM medium at the shoot induction phase and 1.5 mg/ l ppt in the SEM medium at the shoot elongation phase gave the highest selective efficiency. Bacterial solution with OD650 =0.6 with an incubation time of 30 minutes, co-culture for 3 days in the dark, and bactericidal with cefotaxime 500 mg/l is suitable for shoot induction and shoot elongation on selective medium.