QUANTIFICATION OF TOTAL SAPONIN IN GYNOSTEMMA PENTAPHYLLUM WITH 5 LEAVES AND 7 LEAVES COLLECTED IN THAI NGUYEN BY PHOTOMETRIC METHOD

Abstract

Objective: Developing a procedure to quantify total saponins in gynostemma pentaphyllum. therefore, quantitative and comparative the content of total saponin in gynostemma pentaphyllum  with 5 leaves and 7  leaves which were collected in Thai Nguyen by photometric method.

Method: Quantify total saponin in gynostemma pentaphyllum  by photometric method. Extract saponins in medicinal herbs by ultrasonic method with 70% ethanol solvent. Refined with n - buthanol. The total saponin obtained was performed by Rosenthaler reaction, using color solution formed after the reaction to measure photometer. The total saponin content was calculated using the Gypenoside XVII calibration curve.

Conclusion: Applied photometric method to quantify total saponin in  gynostemma pentaphyllum, there was more content of total saponin in gynostemma pentaphyllum with 7 leaves (5.76%) than 5 leaves (4.69%).