PRELIMINARY REPORT ON THE DISTRIBUTION, MORPHOLOGY AND GENETICS OF CUC BONG SOYBEAN IN VO NHAI DISTRIC, THAI NGUYEN PROVINCE

Abstract

Cuc bong is a special soybean variety of Thai Nguyen province. In recent years, the area of cultivating this soybean has been narrowed, facing risk of degeneration and gradual loss of seed variety. Morphological: Cuc bong has a high similarity with DT84 soybean, but the average seed size is smaller, the weight of 1000 seeds ranges from 120-135g, lower than DT84 soybean from 40 -65g. About biochemistry: The protein content of Cuc bong is 36.57%, lipid 21.38%, mineral 4.74%, gluxid 24.37% have less than 17 types of amino acids. In this study, morphological, biochemical and nucleotide sequences of the rbcL and  ITS2 gene segments were analyzed. The results of successful cloning of gene segments (rbcL, ITS2) from leaf tissue of Cuc bong cultivar by PCR technique, with the size of rbcL gene is 700bp and that of ITS2 gene is 500bp. Comparison of the above sequences with the sequences on international Gene Banks showed that the rbcL gene sequence has a similarity of more than 99% for the genus of Glycine genus, the sequence of ITS2 gene has a similarity of 100% with that of Glycine max and 99.7% with Glycine soija (difference 0.3%, and 4-6% for other species belonging to genus Glycine). The nucleotide sequences of ITS2 from Cuc bong have been registered on the NCBI with Barcode ID: MN224216.