BUILDING THE METHOD OF QUANTIFICATION OF TOTAL SAPONIN IN ROOTS OF POLISCIAS FRUTICOSA WHICH WERE HARVESTED AT THAI NGUYEN BY UV-VIS SPECTROPHOTOMETRY

Abstract

Objective: To establish for content determination of total saponins in the roots of poliscias fruticosa. Methods:Total saponins was determined by UV-VIS spectrophotometry after extracts of the samplehad been coloured. Results: The methods was linear in the range of 5-30 µg/ml (r=0.9987), and the average recovery was 99.775%, RSD was 3.5% (n=6) and repetition rate with RSD= 4.74%. Conclusion:The methods is qualified for the determination of the contents of saponins in herbal medicine poliscias fruticosa.