STUDY ON THE SUITABLE MEDIUMS FOR IN VITRO CULTURE THE STEM SEGMENT OF SACHA INCHI (Plukenetia volubilis L.) PLANT

Abstract

From the initial material is seeds, the study identified a suitable in vitro medium for regenerating sacha inchi (Plukenetia volubilis L.) from the stem segment. The seeds of sacha inchi sterilized with 5% NaOCl for 30 minutes give the highest rate of clean germination samples reaching 78.29%. Seeds have the fastest germination rate when sown on MS medium supplemented with 20 g/l sucrose + 7 g/l agar with average tree height of 14.52 cm. Using stem segments between true leaves 1 and real leaves 2 of seedlings in vitro collected from seeds for maximum reproductive regeneration capacity of 72.22%. The stem segment after 4 weeks of growing on MS medium supplemented with 0.1 mg/l BAP and 0.1 mg/l IBA was regenerated 4.67 shoots/sample. These average height of the shoots reached 1.75cm, the number of leaves was 2.50 leaves/shoot. The shoots were then transferred to the rooting medium supplemented with an IBA of 0.5 mg / l, the rooting rate reached 90%, the average number of roots reached 5.85 roots/shoot.