USING DNA BARCODING TO IDENTIFY Anoectochilus setaceus SPECIES COLLECTED AT THUAN CHAU, SON LA, VIET NAM

Abstract

Anoectochilus setaceus is a rare medicinal orchids. Currently, in Viet Nam is known 12 species of Anoectochilus genus, including an Anoectochilus setaceus. Been excessively exploited, that can result in the extinction of Anoectochilus setaceus. Therefore, the collection and species identification to create a basis for storage and propagation contribute to the conservation and development of the Anoectochilus setaceus is very urgent. In this study, we present the results of species identification of Anoectochilus setaceus collected in Thuan Chau, Son La with DNA barcodes based on the sequence of ITS region and rpoC1 gene segment. ITS region isolated from genome DNA of Anoectochilus samples is 666 bp in length and rpoC1 gene is 628 bp in length. Based on nucleotide sequence of ITS region and gene segment rpoC1 and by BLAST software in NCBI, results showed that the Anoectochilus samples collected in Thuan Chau, Son La were identified as Anoectochilus setaceus species.