OPTIMIZATION OF RECOMBINANT CsLAR1 EXPRESSION IN E. COLI ROSETTA AND ACTIVITY ANALYSIS

Abstract

CsLAR1 gene isolated from the TrungDuxanh tea has grown in Thai Nguyen, It was ligated to pET32a(+) vector  and expressed in E. coli Rosetta bacteria. This study presents the optimal results of expression and activity analysis of recombinant CsLAR1 (rCsLAR1). E. coli strain containing pET32a(+)_CsLAR1 produces a large amount of rCsLAR1 when cultured in an LB supplemented with ethanol at 16 ^oC, after inducing 24 hours. rCsLAR1 is incubated in an extract solution from fresh tea leaves that alters the content of some substances compared to the control but not catechin, gallocatechin or epicatechin. The rCsLAR1 activity was obtained, demonstrating that CsLAR1 can catalyze other substrate in tea in addition to leucocyanidins.