THE EXPRESSION OF GENE AQUAPORIN 1 (AQP1) ON ANABAS TESTUDINEUS EXPERIMENTALLY GROWN IN SALT WATER

Abstract

The complete cDNA sequence encoding AQP1 obtained from the gills of Anabas testudineus consists of 786 nucleotides that have been granted accession number MT012828 by GenBank USA, encoded for AQP1 with 261 amino acids which have an estimated molecular weight of 27.4 kDa. The expression level of AQP1 in Anabas testudineus gills living in salt water (salinity 30) after 6 days was the highest with 12.26´10² copies/ng cDNA, the skin of fish was 10.65´10² copies/ng cDNA, the kidneys of fish was 2.82´10² copies/ng cDNA and the lowest was in the gut of fish 0.48´10² copies/ng cDNA. The AQP1 expression level when Anabas testudineus lived in salt water (salinity 30) after a day was highest in gill of fish 7.12´10² copies/ng cDNA, followed was in the skin and kidneys of fish 4.13´10² copies/ng cDNA and 4.47´10² copies/ng cDNA, the lowest was in the gut of fish 0.27´10² copies/ng cDNA