ẢNH HƯỞNG CỦA VIỆC BỔ SUNG HUYẾT THANH THAI BÒ TRONG MÔI TRƯỜNG NUÔI CẤY ĐẾN SỰ PHÂN LẬP VÀ TĂNG SINH TẾ BÀO NGUYÊN SỢI TỪ MẪU MÔ BUỒNG TRỨNG HEO Lưu Khải Nhiên1 và Nguyễn Ngọc Tấn *

Lưu Khải Nhiên1 và Nguyễn Ngọc Tấn1*

Abstract

Effect of Fetal bovine serum supplementation in culture media on isolation and proliferation

of fibroblast cells from porcine ovarian tissue

The aim of this study was to investigate to effect of fetal bovine serum (FBS) supplemented in culture medium for fibroblast cells from porcine ovarian tissue. Tissue explants were submitted to primary culture in medium with different concentration of FBS for 8 days with a medium change at day 4. Results shown that between the two treatments of supplementing 10 and 20% FBS throughout 8 days, a significant difference (5.01 and 5.57; P<0.05) of cells harvested was found. No differences of harvested cell numbers were observed between supplementing 10-20 and 20-10% at first 4 days - later 4 days (5.46 and 5.40, respectively). Three levels of FBS concentration (10, 15 and 20%) were evaluated for subculture medium, proliferative activity of cells were recorded to build a growth curve and determine population doubling time (PDT). There were significant differences (P<0.05) between 10 and 20% FBS supplementation in culture medium on day 3 (4.04 and 4.24, respectively), day 5 (4.24 and 4.45, respectively) and day 7 (4.19 and 4.41, respectively). No differences were recorded between the 15 and 20% treatments. The PDT of 10, 15 and 20% treatments was 68.89, 66.65 and 62.04 hours, respectively. The growth curve with the exponential phase spanning from day 1 to day 5 was determined. In conclusion, a supplementation of 10% FBS in the first 4 days and 20% FBS in the later 4 days of primary culture medium and subculture in medium with 15% FBS enhanced the total number of fibroblast cells isolated and proliferated from porcine ovarian tissue.