SCREENING AND SELECTING LACTIC ACID BACTERIA STRAIN RESISTING METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) has been described as having a rapid response to each new class of antibiotics with the development of a resistance mechanism, from penicillin and methicillin to vancomycin and teicoplanin, and, to the nearest, linezolid and daptomycin. In this study, from lactic acid bacteria (LAB) strains, strains having the most effective resistance to MRSA were selected by the wells diffusion method. From the selected strains, analogizing antibacterial factors was applied to investigate its mechanism. The results show that bacteriocin plays a key role in antibacterial fluid. A strain with the strongest activity in the selected strains was used to study the effectiveness between the selected strain and MRSA by culturing in a simulated gastric juice medium on the BIOFLO fermenter system with the same condition as human stomach. Observations under electron microscopy shows that this strain is capable of inhibiting MRSA biofilm formation. The selected strain was identified as Lactobacillus rhamnosus.