Isolating and investigating the effects of the nutrient salts (NO3-, PO43-) on the development of green algae Chlorella sp.

Abstract

Chlorella is a fast growth and long-life green algae, therefore, it is suitable to obtain the good and stable water color in aquaculture ponds. After Chlorella was isolated, experiments were carried out to investigate their potential for growth and stability shown during the development cycle. The experiments were conducted to evaluate the effects of some nutrient salts including NO₃^-, PO₄^3- at different concentrations in the laboratory. The results showed that chlorella was the fastest growth in NO₃^- = 1,2mg/L; PO₄^3- = 0,5mg/L concentration, and reached the maximum amount at (8,93 ± 2,5)x10⁶ tb/m Land stable growth period in NO₃^- = 1,2mg/L; PO₄^3- = 0,25mg/L concentration for 21 days of culture. Two-way ANOVA showed no significant difference in growth and life cycle between experimental trials (P ≥ 0.05) with different levels of NO₃^-, PO₄^3-

            Keywords: Chlorella sp., life cycle, culture, micro-algae