Effects of demecolcine and oocyte maturation time on the efficiency in the production of Vietnamese pot-bellied pig embryos

  • Thi Au Hoang, Thi Kim Yen Pham, Thi Thu Huong Vu, Van Dat Le, Thi Lan Anh Nguyen, Thi Thanh Nhan Giang, Khanh Van Nguyen*
  • Doan Lan Pham
  • Quang Minh Luu
Keywords: Demecolcine, maturation in vitro, Vietnamese pot-bellied pig embryos.

Abstract

The results of this study showed that the concentration of Demecolcine affected efficiency in the production of Vietnamese pot-bellied pig embryos. The 4 μM Demecolcine group resulted in a greater oocyte extrusion cones rate in comparison to 0, 6, and 8 μM groups (72.03 vs 1.98%, 59.42, and 51.01%, p<0.05, respectively). The cleavage rate and blastocyst rate in the 4 μM Demecolcine group were significantly higher than in the 6 and 8 μM groups (p<0.05)...

Tác giả

Thi Au Hoang, Thi Kim Yen Pham, Thi Thu Huong Vu, Van Dat Le, Thi Lan Anh Nguyen, Thi Thanh Nhan Giang, Khanh Van Nguyen*

Key Laboratory of Animal Cell Technology, National Institute of Animal Science, 9 Tan Phong Street, Thuy Phuong Ward, Bac Tu Liem District, Hanoi, Vietnam

Doan Lan Pham

National Institute of Animal Science, 9 Tan Phong Street, Thuy Phuong Ward, Bac Tu Liem District, Hanoi, Vietnam

Quang Minh Luu

Ministry of Science and Technology, 113 Tran Duy Hung Street, Trung Hoa Ward, Cau Giay District, Hanoi, Vietnam

điểm /   đánh giá
Published
2023-12-25