Characterization of multi-antibiotic resistant bacteria isolated from climbing perch (Anabas testudineus) and therapeutic potential of phytochemicals

Abstract

This study was conducted to investigate the characteristics of bacteria isolating from the diseased climbing perches, including in-vitro antimicrobial susceptibility test and virulent examination. A total of 30 bacterial strains, including 10 strains of Vibrio and 20 strains of nonVibrio were isolated in this study, they resisted to 9 experimental antibiotics. There were 11 out of 12 investigated strains resisted to at least five antibiotics. All of 12 these bacteria strains were susceptible to ciprofloxacin, but this antibiotic was presently prohibited to use in aquaculture in Viet Nam, and in the result of experimental infection for climbing perch fingerlings with the above bacteria strains with a dose of 105 CFU/fish showed that 12 these isolates caused mortality of
fish with the rate varying from 40-90%. The result of testing bacterial inhibition of phytochemicals showed that TT1 and TT2 substances resisted to 2 highest virulent strains, such as: NV5M and V7L. The results of this study also indicated that the use of antibiotics in prophylaxis and therapy
for fish should be controlled more strictly. The use of phytochemicals replacing antibiotics in aquaculture would be a safe solution. Further studies on molecular (antibiotic resistant gene, plasmid of bacteria) are necessary in order to improve the efficacy of controlling antibiotic use
in aquaculture, to reduce environmental pollution so as to protect human health