Development of loop mediated isothermal amplification assay for detecting PRRSV, CSFV, PEDV and PCV2

Abstract

In this study, we developed loop-mediated isothermal amplification (LAMP) assay targeting the ORF-6 gene for the detection of PRRSV, the NS5B gene for the detection of CSFV, the M gene for the detection of PEDV and the ORF-2 gene for the detection of PCV2. This amplification method could be obtained in 1 hr. under isothermal conditions (630C) employing a set of four specific primers mixtures. The sensitivity was 0.1ng/µl and no cross-reactivity for different recombinant plasmids.