Determining some pathogenic genes of heating resistant Campylobacter spp. isolated from chicken meat and pork in Viet Nam

Abstract

The result of this study showed that nine Campylobacter isolates (eight C. jejuni and one C. coli) from 20 strains were identified virulen gene by multiplex PCR, lipooligosaccharide (LOS) biosynthesis and further functions was tested. In all isolates carrying the motility flagella genes (flaA, flaB, flhA, fliM), colonization associated genes (cadF, docB), toxin production genes (cdtA, cdtB, secD, secF), and the LOS biosynthesis gene pglB were detected. Meanwhile, eight gene loci (fliY, virB11, Cje1278, Cj1434c, Cj1138, Cj1438c, Cj1440c, Cj1136) were not identified by PCR. However, there was the difference of the gene loci ciaB (22.2 %), Cje1280 (77.8 %), docC (66.7 %), and cgtB (55.6 %) found. The gene iamA, cdtC were identified in all C. jejuni isolates but not in C. coli.