Phân lập virus dịch tả lợn Châu Phi trên môi trường tế bào Porcine Alveolar Macrophages (PAM)

Abstract

In this study, the porcine alveolar macrophages (PAM) cell medium was used for culturing and isolating the African swine fever virus (ASFV) from the field samples. The ASFV suspected pigs with the typical clinical signs and pathological lesions, was used as the studied material. The ASFV was detected by conventional PCR technique in combination with sequence analysis using specific primers, PPA1 and PPA2. As a result, the samples were positive with ASFV by PCR and ASFV strain presented 100% of the nucleotide sequence similarity level in comparison with those of the previous isolation ASFV. Furthermore, the isolation of ASFV was conducted using PAM cell system and this isolate was further confirmed by haemadsorption (HAD) test and PCR technique according to the recommendation of OIE/FAO. The studied result showed that the isolated virus strain was positive in both HAD test and PCR technique. It is concluded that ASFV was successfully isolated on PAM cells from the field samples in Viet Nam