Isolation and genetic analysis of Canine distemper virus (CDV) in domestic dogs in Ho Chi Minh City, Viet Nam

Abstract

CDV is one of the most serious pathogens found in many carnivorous animal species, including
domestic dogs. In this study, H gene of CDV were detected in five domestic dogs, Viet Namese breed,
suffering with diarrhea. There were two, out of the identified CDVs from the positive dogs with H genes
successfully isolated. The complete genome of one isolated virus, CDV/dog/HCM/33/140816, was
determined. The result of phylogenetic analysis showed that all the Viet Namese CDVs belonged to
the Asia-1 genotype. In addition, the result of analyzing the isolated CDVs indicated the H proteins of
Viet Namese CDV strains were highly similar in comparison with those of the Chinese CDVs (98.4%
to 99.3% identity). These results indicated that the Asia-1 genotype of CDV was the predominant
genotype circulating among the domestic dog population in Ho Chi Minh City. It is indicated that
transboundary transmission of CDVs may be occurred between Viet Nam and China.