Molecular epidemiology of Canine Parvovirus from domestic dogs in Ho Chi Minh City

Abstract

CPV-2 is one of the most important pathogens causing enteritis in domestic dogs. Although
there were some reports of CPV in Viet Nam, recent information on CPV infection in domestic
dogs in Ho Chi Minh City is limited. To examine the prevalence of CPV-2 in the domestic dog
herds, fecal samples from 30 diarrheic and 50 healthy dogs were collected and detected for CPV-
2 by PCR technique. The studied result showed that the prevalence of CPV in the diarrheic dogs
was 43.3% (13/30), significantly higher than that in the healthy dogs (4.0%, 2/50), indicating that
CPV-2 was a pathogen causing diarrhea in the domestic dogs. The result of genotyping 15 CPV
strains showed that the New CPV-2a and CPV-2c were circulating in Ho Chi Minh City and CPV-
2c was a predominant genotype causing diarrheic disease. Virus isolation was performed from
the fecal samples by using A72/cSLAM cells, and nine CPV strains were successfully isolated.
Genetic analysis showed that mutations in VP2 protein were found in Viet Namese CPV-2c (A5G,
Y324I, F267Y, Q370R) and New CPV-2a (Y324I, F267Y, T440A). These mutations might affect
to antigenicity and pathogenicity of them.