Optimization of Polyphenol Extraction from Guava Leaves by Response Surface Methodology
Abstract
This study was carried out to optimize extraction condition of polyphenols from guava leaf. To obtain this goal, we used a response surface methodology to optimize extracting conditions of polyphenols. Four main factors affecting on polyphenol extraction conditions from guava leaf were investigated, including: Temperature, time, solvenmaterial ratio, and solvent concentration. Research results showed that the optimal extraction conditions of polyphenols from guava leaf obtained as follow: Temperature of 90oC, time of 76.5 mins, a solvent-material of 70/1 (ml/g), and a ethanol concentration of 44.3%. At optimal extraction conditions, polyphenol content achieved 233.76 mg GAE/g of dry basis. Polyphenol extract from guava leaf obtained at the optimal condition exhibited high antioxidant activity based DPPH free radical scavenging ability with an IC50 value of 2.1 μg/ml. Our research results indicated potential of using guava leaf as promisisng resources for extracting natural antioxidants.