Adventitious Root Induction and Culture of Morinda officinalis How

Abstract

      This paper presents the results on induction of adventitious roots of Morinda officinalis How and effect of some chemical factors on adventitious root growth. Stem and leaf explants were cultured on MS medium supplemented with α-NAA, IAA and IBA at five concentrations (0.1-1.0 mg/l) to regenerate adventitious roots. Among the auxins, α-NAA showed the highest adventitious root formation for stem explants, followed by IAA and IBA. The maximum percentage of stem explants induced root (100%) was obtained when cultured on MS medium supplemented with 0.75 mg/l α-NAA after 4 weeks of culture. However, adventitious root induction was not observed in leaf explants cultured on MS + 0.75 mg/l α-NAA. Of the two tested media, B5 medium sustained better root growth than MS medium. α-NAA, IAA and IBA had positive effect on adventititous root growth and development. After 12 weeks, the highest adventitious root biomass (1.264 g) was obtained on B5 medium supplemented with 1.0 mg/l α-NAA.