STUDY OF ADVENTITIOUS ROOT FORMATION DERIVED FROM NODE OF Morinda officinalis How. CULTURED IN VITRO

Abstract

Morinda officinalis is a precious medicinal plant with high economic value in Vietnam. In recent years, along with the tendency of breeding and preserving medicinal plants, the cultivation of biomass roots has been receiving attention. In this study, the role of IBA (indole-3-butyric acid), method of placing node on root induction were investigated. The results showed that SH (Schenk & Hildebrandt) medium supplemented with 2 mg/L IBA was suitable for adventitious root induction of Morinda officinalis cultured in vitro. The node explant placed vertically obtained the higher ratio of root formation (100%) and numbers of root (13.46 roots/sample) than node explant placed horizontally. The state of medium culture and the concentration of sucrose also play an important role in the proliferation of adventitious root. The results indicated that explants cultured on solid SH medium (agar 8.0 g/L) had higher growth rates than explants cultured on shaking liquid medium (without agar). In the experiment using sucrose, the medium supplemented with 45 g/L sucrose was suitable for adventitious root proliferation. The fresh weight of adventitious root achieved the highest value which was six times higher than initial used weight (1,2 g). The results of the study can be referred for the process of culturing Morinda officinalis root biomass at large scale, providing materials for the pharmaceutical industry.