DEVELOPING METHOD FOR QUICK IDENTIFICATION OF Cry1Ab- GENETICALLY MODIFIED MAIZE BASED ON LAMP TECHNIQUE

Abstract

Vietnam has allowed the cultivation of gentically modified maize containing Cry1Ab gene to increase resistance to stem borer. Despite many advantages, there are still many debates about the safety of this crop so there are many methods used to detect them. However, the current methods rely heavily on laboratory equipment and are difficult to perform in the field. Recently, the LAMP (Loop Mediated Isothermal Amplification) DNA amplification technique has been used to quickly detect human and animal disease objects, with high accuracy and less requirements for expensive equipment. Within the scope of this study, we have successfully developed a process to detect gentically modified maize using LAMP technology. The results of the study have optimized parameters for LAMP reaction including concentration of FIP/BIP and F3/B3 of 0.4 µM with primer pairing temperature at 60 °C. The authors also found that the sensitivity of LAMP reaction was better than that of Realtime PCR in studied range. When using HNB dye at a concentration of 80 nM, it was possible to distinguish between the non-transgenic and Cry1ab gene containing maize. This result is a potential for designing KIT to detect gentically modified corn in the field.

Keywords: Corn, Cry1Ab, GMO, LAMP, Realtime PCR.