INVESTIGATION OF PROTEIN PRECIPITATION OF EXTRACT FROM Chaetomorpha sp. USING ACID AND ETHANOL

Abstract

Currently, the research and development of processes for obtaining protein from different materials are increasingly focused on, and bring about many benefits. Chaetomorpha sp. is one of the potential ingredients with a high protein content, but it is removed as waste. In this study, the factors affecting the efficiency of protein precipitation and the protein purity after precipitation with acid and ethanol such as extract-to-solvent ratio, solvent content, pH, temperature and duration of protein precipitation were investigated in order to improve the efficiency of protein production from Chaetomorpha sp. The method of protein precipitation with acid gave the highest efficiency of protein recovery of 73.48% and the protein purity of 41.30% under the optimal conditions (pH 3, temperature of 35 °C and treatment time of 90 minutes); while for the protein precipitation with ethanol, the protein recovery of 59.24% and the protein purity of 55.08% were achieved under conditions including the extract-to-solvent ratio of 1:4, ethanol 90%, treatment time of 50 minutes at 5 °C. 

Keywords: Acid, Chaetomorpha sp., ethanol, protein precipitation, protein concentrate/isolate.