CHEMICAL COMPOSITION AND OSTEOPOROSIS ACTIVITY FROM ETHYL ACETATE EXTRACT OF CLINACANTHUS NUTANS

Abstract

Phytochemical studies on ethyl acetate extract of Clinacanthus nutans led to
three compounds, myricitrin (1), myricetin (2), and lupeol (3) were isolated.
Their structures were determined based on NMR and MS spectra, as well as a
comparison with the NMR data reported in the literature. In this study, bone
regeneration activity of compounds was assessed using an in vitro model of
osteoblast cells MC3T3-E1. The finding revealed that the compounds lupeol
demonstrated the osteogenic activity through enhancement of alkaline
phosphatase (ALP) of osteoblast cells up to 31.2%, 21% and 12% at
concentrations of 5, 10 and 20µg/mL and the mineralization activity was
increased up to 170, 230, 185, and 117% at concentration of 5, 10, 20 and
40μg/mL, respectively (p < 0.05).