EVALUATION OF POTENTIAL TARGETING IN VITRO TO COLORECTAL CANCER CELL FROM NANO Tb3+ - MONOCLONAL ANTIBODY (RT) TRANSPORT SYSTEM

Abstract

Colorectal cancer is a major global health problem with an annual incidence of 1.7 million worldwide [1]. Around
6.3 million people in the world live with colorectal cancer which is the second most diagnosed cancer estimated to
have caused around 860,000 deaths in 2018 [2]. Conventional treatments of colon cancer have left several undesired
aftereffects. In order to limit these side effects and give relief for the patient, it is necessary to have a targeted
transport system highlighting colorectal cancer cells for localization before chemo- and radiotherapy. This study aims
to evaluate the target transport (ET) system through RT marking with high-yield on colorectal cancer cells over
normal cells. The study used variable infrared spectroscopy system (Fourier Impact 410 NICOLET) to determine the
luminescence of transport system, flowcytometry to determine the number of marked cells. The results demonstrated
that the ET transport system in vitro gave marks on 26.89% of colorectal cancer cells compared to the control, while
they did not pinpoint healthy cells. It lays the basis for a new targeting approach in the treatment of colorectal cancer
to meet a demand for finding new fluorescence nanomaterials to control the deadly disease nowadays.