Định danh nấm beauveria bassiana ký sinh côn trùng gây hài ở một só tỉnh Đồng bằng sông Cửu Long bằng phương pháp pcr, thử hiệu lực của nấm này và một số thuốc trừ sâu đối với sâu ăn tạp (spodoptera litura fabricius) hại rau

Abstract

The study was carried out at the laboratory  Microbiology, Mekong University to confirm the identification of Beauveria bassiana fungi collected from vegetable crops in some provinces of the Mekong delta using PCR technique, Beauveria bassiana isolates were selected and some pesticides (chemical, biological and botanical plants) effective control of pests worm omnivorous (Spodpera litura Fabricius). The result has been isolated 6 Beauveria bassiana parasitic on insects in 6 provinces of An Giang, Dong Thap, Vinh Long, Can Tho, Tien Giang and Hau Giang. In particular, the isolates were tested by PCR with primers Bebas spF- Bebas spR. The PCR product of 6 isolates Bb - AG, Bb - ĐT, Bb - VL, Bb - CT, Bb - HG, Bb - TG is the color band size of 540 kp was  identified Beauveria bassiana Vuill. The survey showed that isolates effect Bb - AG effective 3 days after spraying.