Development and validation of a method for quantification of vicenin 2 in Glinus oppositifolius (L.) DC. Molluginaceae
Abstract
Vicenin-2 is a natural, bio-active flavonoid extracted from several plant species including Glinus oppositifolius. Recent studies have shown that Vicenin-2 has anti-inflammatory activity, hepatoprotection, hypoglycemic, anti-cancer, … Research has been conducted to develop and validate Vicenin 2 in Glinus oppositifolius with HPLC-PDA while validating the standard of the determination process, thereby proposing a new to determine Vicenin-2 in Glinus oppositifolius. The procedure was performed with Sunfire C18 Column (4.6 × 150 mm, 5 µm), and using the mixture of 0.1 % CH3CN–HCOOH as a mobile phase. The UV detection was performed at 335 nm. “Vietnamese Pharmacopoeia V” has a monograph on Glinus oppositifolius, but there is no quantitative criterion, so this will be the premise to develop a process to quantify the active ingredients in this medicinal herb. The procedure has the potential to be widely utilized in our nation, and contribute to bringing modern testing procedures for Glinus oppositifolius quality control in particular, along with herbs and herbal medicines in general.
