Nanochitosan acts as a growth promotor in orchid plant (Dendrobium anosmum Lindl, 1845)

Abstract

This study presents the best concentration and spraying schedule of the nanochitosan solution for pretreatment and culture of Dendrobium anosmum Lindl, 1845 in vitro. The baby orchids have a hight of 1.5 – 4.0 cm, 2 – 5 leaves, and a minimum 2 roofs. The pretreated orchids were attached to the pretreated substrates and located in a garden with proper conditions, such as the sunlight, moisture, temperature. The results show that the best conditions include three steps (không có trên bản tiếng Việt): (i) soaking the substrate in 50 ppm nanochitosan solution for 14 h, (ii) soaking the initial orchids 20 ppm nanochitosan solution for 22 min, (iii) spraying 20 ppm nanochitosan solution for one per 8 days or 12 days. This study proposes the use of a biomaterial (bio-product or bio-formulation) and its loadings (application?) in the culture of Dendrobium anosmum Lindl in vitro.