PHÁT TRIỂN PHƯƠNG PHÁP SẮC KÝ LỎNG KHỐI PHỔ HAI LẦN XÁC ĐỊNH HÀM LƯỢNG MỘT SỐ HỢP CHẤT PER- VÀ POLYFLUOROALKYL (PFAS) TRONG THỰC PHẨM

Tóm tắt

PFAS are a large, complex group of synthetic chemicals that are highly resistant to degradation in the
environment. They may cause cancer and adverse effects on the reproductive and immune systems. In this
study, 16 PFAS compounds were determined in food samples (fish, meat, vegetables) using the LC-MS/MS
method to assess contamination levels and support food safety risk control. A reversed-phase C18
chromatography column (150 mm × 2.1 mm; ID 3.5 µm) was used to separate the 16 compounds. The
mobile phase consisted of (A) 2 mM ammonium acetate (CH3COONH4 )/methanol (MeOH) (9/1, v/v) and
(B) MeOH. The extraction solvent was 1% formic acid (HCOOH)/acetonitrile (ACN), and the extraction salt
compositions contained 6 g MgSO₄ combined with 2 g NaCl. Samples were cleaned up by d-SPE
composition of 1.2 g MgSO₄ combined with 0.2 g PSA and 0.2 g C18, and SPE using Polymeric Weak Anion
SPE cartridge. The calibration curves for the 16 substances, within a concentration range of 0.1–5.0 µg/kg,
showed correlation coefficients (R²) greater than 0.995. The method achieved recoveries ranging from
75.7% to 110%, with relative standard deviations (RSDs) between 1.46% and 9.72%. The method detection
limit (MDL) and method quantitation limit (MQL) were 0.03 µg/kg and 0.1 µg/kg, respectively. This method
was applied to the analysis of 20 food samples, and the results showed that 5 of these samples tested positive
for specific PFAS compounds. This study may lay the foundation for developing quantitative procedures for
PFAS groups in various food matrices.