DEVELOPMENT OF A METHOD FOR ISOLATION AND SEQUENCING OF ASPERGILLUS FLAVUS AND ASPERGILLUS PARASITICUS FROM GROUNDNUT

Abstract

   Aflatoxins is mycotoxin commonly contaminating in agricultural commodities,especially in maize and groundnut. Aflatoxins can cause liver cancer, cell carcinoma,reduce immune system and increas stunting in child. Aflatoxins are toxic metabolitesprimarily produced by molds, mainly: Aspergillus flavus and Aspergillus parasiticus.Those species of fungi are similar in morphology causing difficulties in defining andclassifying. This study combined two techniques including isolation based onmorphology and sequence ITS region. Whole 30 strains identified from peanut bymorphology technique, including two groups Aspergillus flavus and Aspergillusparasiticus. From 30 obtained strains we conducted sequence based on Sangertechnique and practised scanning electron microscopy to confirmed 03 strains ofAspergillus flavus and 01 strain of Aspergillus parasiticus.